Hungary is northernmost temperate rice growing country in Europe. One of the main limiting factors is low temperature, especially at germination and seedling developmental stages.
In early developmental stages, low temperature can impair and delay germination, as well as have negative impacts on seedling growth, causing poor stand establishment and non-uniform crop maturation [1]. Temperatures lower than 15 °C are generally detrimental for germination
[2] under filed conditions for establishment of the crop.
This article describes some key germination parameters of 165 rice accessions including breeding lines and varieties. Each genotype was grown in three replicates in a controlled cabinet under 13 °C for 4 weeks’ duration. Growth was measured every 7th day.
Growth traits such as coleoptile and radicle length were measured at the end of the experiment. The average data were calculated for three replicates.
This dataset contains germination raw data and five germination parameters such as median germination time (MGT), final germination percentage (FGP), germination index (GI), coleoptile length (CL) and radicle length (RL). These data may provide reliable support for researchers and breeders to select the right rice genotypes for low temperature conditions.
ZmPRD1 is essential for DSB formation, but not required for bipolar spindle assembly during maize meiosis.
Homologs of PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) are essential for meiotic double-strand break (DSB) formation in mouse (Mus musculus), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa).
Latest work reveals that the rice PRD1 also plays an unanticipated role in meiotic bipolar spindle assembly, highlighting multiple functions of PRD1 in plant meiosis.
In this work, we characterize the meiotic function of PRD1 in maize (Zea mays; ZmPRD1). The Zmprd1 mutant plants display normal vegetative growth but complete male and female sterility.
Meiotic DSB formation is fully abolished in mutant meiocytes, leading to the failure in homologous pairing, synapsis, and recombination.
ZmPRD1 exhibits distinct pattern of chromosome localization as its rice homologs, and interacted with several DSB-forming proteins, but not directly interacts with kinetochore protein REC8 and SGO1.
Possibly as a result, there are no significant abnormalities of bipolar spindle assembly in Zmprd1 meiocytes. Overall, our results demonstrate that ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis.
However, the newly-identified function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in term of different plant species.
Label-free proteomics approach reveals candidate proteins in rice (Oryza sativa L.) important for ACC deaminase producing bacteria-mediated tolerance against salt stress.
- The omics-based studies are important for identifying characteristic proteins in plants to elucidate the mechanism of ACC deaminase producing bacteria-mediated salt tolerance. This study evaluates the changes in the proteome of rice inoculated with ACC deaminase producing bacteria under salt stress conditions.
- Salt stress resulted in a significant decrease in photosynthetic pigments, whereas inoculation of Methylobacterium oryzae CBMB20 had significantly increased pigment contents under normal and salt stress conditions. A total of 76, 51 and 33 differentially abundant proteins (DAPs) were identified in non-inoculated salt stressed plants, bacteria inoculated plants under normal and salt stress conditions, respectively.
- The abundances of proteins responsible for ethylene emission and programmed cell death were increased, and that of photosynthesis-related proteins were decreased in non-inoculated plants under salt stress.
- Whereas, bacteria-inoculated plants had shown higher abundance of antioxidant proteins, RuBisCo and ribosomal proteins that are important for enhancing stress tolerance and improving plant physiological traits.
- Collectively, salt stress might affect plant physiological traits by impairing photosynthetic machinery and accelerating apoptosis leading to a decline in biomass. However, inoculation of plants with bacteria can assist in enhancing photosynthetic activity, antioxidant activities and ethylene regulation related proteins for attenuating salt induced apoptosis and sustaining growth and development. This article is protected by copyright. All rights reserved.
miR2105 and the kinase OsSAPK10 co-regulate OsbZIP86 to mediate drought-induced ABA biosynthesis in rice.
- Mediating induced abscisic acid (ABA) biosynthesis is important for enhancing plant stress tolerance. Here, we found that rice (Oryza sativa L.) osa-miR2105 (miR2105) and the Stress/ABA-activated protein kinase (OsSAPK10) coordinately regulate the rice bZIP transcription factor (OsbZIP86) at the post-transcriptional and post-translational levels to control drought-induced ABA biosynthesis via modulation of rice 9-cis-epoxycarotenoid dioxygenase (OsNCED3) expression.
- OsbZIP86 expression is regulated by miR2105-directed cleavage of the OsbZIP86 mRNA. OsbZIP86 encodes a nuclear transcription factor that binds to the promoter of the ABA biosynthetic gene OsNCED3. OsSAPK10 can phosphorylate and activate OsbZIP86 to enhance the expression of OsNCED3.
- Under normal growth conditions, altered expression of miR2105 and OsbZIP86 displayed no substantial effect on rice growth.
- However, under drought conditions, miR2105 knockdown or OsbZIP86 overexpression transgenic rice plants showed higher ABA content, enhanced tolerance to drought, lower rates of water loss, and more stomatal closure of seedlings, compared with wild-type rice Zhonghua 11 (ZH11); by contrast, miR2105 overexpression, OsbZIP86 downregulation, and OsbZIP86 knockout plants displayed opposite phenotypes.
- Collectively, our results show that the ‘miR2105-(OsSAPK10)-OsbZIP86-OsNCED3’ module regulates the drought-induced ABA biosynthesis without penalty on rice growth under normal conditions, suggesting candidates for improving drought tolerance in rice.
Genome-wide characterization of i-motifs and their potential roles in the stability and evolution of transposable elements in rice.
- I-motifs (iMs) are non-canonical DNA secondary structures that fold from cytosine (C)-rich genomic DNA regions termed putative i-motif forming sequences (PiMFSs).
- The structure of iMs is stabilized by hemiprotonated C-C base pairs, and their functions are now suspected in key cellular processes in human cells such as genome stability and regulation of gene transcription. In plants, their biological relevance is still largely unknown.
- Here, we characterized PiMFSs with high potential for i-motif formation in the rice genome by developing and applying a protocol hinging on an iMab antibody-based immunoprecipitation (IP) coupled with high-throughput sequencing (seq), consequently termed iM-IP-seq.
- We found that PiMFSs had intrinsic subgenomic distributions, cis-regulatory functions and an intricate relationship with DNA methylation.
- We indeed found that the coordination of PiMFSs with DNA methylation may affect dynamics of transposable elements (TEs) among different cultivated Oryza subpopulations or during evolution of wild rice species.
- Collectively, our study provides first and unique insights into the biology of iMs in plants, with potential applications in plant biotechnology for improving important agronomic rice traits.
Unravelling the physiological basis of salinity stress tolerance in cultivated and wild rice species.
Wild rice species provide a rich source of genetic diversity for possible introgression of salinity stress tolerance in cultivated rice. We investigated the physiological basis of salinity stress tolerance in Oryza species by using six rice genotypes (Oryza sativa L.) and four wild rice species.
Three weeks of salinity treatment significantly (P<0.05) reduced physiological and growth indices of all cultivated and wild rice lines.
However, the impact of salinity-induced growth reduction differed substantially among accessions. Salt tolerant accessions showed better control over gas exchange properties, exhibited higher tissue tolerance, and retained higher potassium ion content despite higher sodium ion accumulation in leaves.
Wild rice species showed relatively lower and steadier xylem sap sodium ion content over the period of 3weeks analysed, suggesting better control over ionic sodium xylem loading and its delivery to shoots with efficient vacuolar sodium ion sequestration.
OryzaExp? Human Serum Albumin |
|||
| P1396-1 | Biovision | each | 523.2 EUR |
OryzaExp? Lysozyme, Human Recombinant |
|||
| P1400-1 | Biovision | each | 523.2 EUR |
OryzaExp? Fibronectin, Human Recombinant |
|||
| P1401-100 | Biovision | each | 574.8 EUR |
OryzaExp? Lactoferrin, Human Recombinant |
|||
| P1403-1 | Biovision | each | 520.8 EUR |
OryzaExp? FGF-basic, Human Recombinant |
|||
| P1397-50 | Biovision | each | 548.4 EUR |
OryzaExp? IGF-1 LR3, Human Recombinant |
|||
| P1399-1 | Biovision | each | 1057.2 EUR |
OryzaExp? IGF-1 LR3, Human Recombinant |
|||
| P1399-100 | Biovision | each | 210 EUR |
OryzaExp? Alpha-1 Antitrypsin, Human Recombinant |
|||
| P1402-100 | Biovision | each | 510 EUR |
OryzaExp? Epidermal Growth Factor, Human Recombinant |
|||
| P1398-50 | Biovision | each | 535.2 EUR |
Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf) |
|||
| 1-CSB-EP2860XBC | Cusabio |
|
|
Recombinant Xanthomonas oryzae pv. oryzae Enolase (eno) |
|||
| MBS1223563-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | 1300 EUR |
Recombinant Xanthomonas oryzae pv. oryzae Enolase (eno) |
|||
| MBS1223563-002mgEColi | MyBiosource | 0.02mg(E-Coli) | 980 EUR |
Recombinant Xanthomonas oryzae pv. oryzae Enolase (eno) |
|||
| MBS1223563-002mgYeast | MyBiosource | 0.02mg(Yeast) | 1095 EUR |
Recombinant Xanthomonas oryzae pv. oryzae Enolase (eno) |
|||
| MBS1223563-01mgEColi | MyBiosource | 0.1mg(E-Coli) | 1145 EUR |
Recombinant Xanthomonas oryzae pv. oryzae Enolase (eno) |
|||
| MBS1223563-01mgYeast | MyBiosource | 0.1mg(Yeast) | 1245 EUR |
Recombinant Xanthomonas oryzae pv. oryzae GTPase Era (era) |
|||
| MBS1353182-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | 1180 EUR |
Contrary to this, saline sensitive genotypes managed to avoid initial Na+ loading but failed to accomplish this in the long term and showed higher sap sodium ion content.
Conclusively, our results suggest that wild rice genotypes have more efficient control over xylem sodium ion loading, rely on tissue tolerance mechanisms and allow for a rapid osmotic adjustment by using sodium ions as cheap osmoticum for osmoregulation.